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Study #1: PATN1 + LPP1/LPP2

This study looks at how LPP1 and LPP2 interact with PATN1 when Varnish Promoters are present, and Darkening Genes are added. 

Bay_HOMPatn1_lpp1_lpp2_VRN.jpg
Study #1: 8yo Bay Appaloosa Mare LPP1/LPP2
E/E, A/A, f/-, P/P, W20/-, LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn

Removing LP Promoter Genes

Bay_HOMPatn1_lpp1_lpp2_VRN.jpg

LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn

Bay_HOMPatn1_lpp2_VRN.jpg

LP/-, PATN1/PATN1, LPP2/-, Vrn/Vrn

  

Bay_HOMPatn1_lpp1_VRN.jpg

LP/-, PATN1/PATN1, LPP1/-, Vrn/Vrn

Bay_HOMPatn1_VRN.jpg

LP/-, PATN1/PATN1, Vrn/Vrn

Notice how she went from Leopard to Near Leopard with the removal of LP Promoter Genes (LPP1 and LPP2)

Heterozygous for PATN1

Bay_HETPatn1_lpp1_lpp2_VRN.jpg

LP/-, PATN1/-, LPP1/LPP2, Vrn/Vrn

Bay_HETPatn1_lpp2_VRN.jpg

LP/-, PATN1/-, LPP2/-, Vrn/Vrn

Bay_HETPatn1_lpp1_VRN.jpg

LP/-, PATN1/-, LPP1/-, Vrn/Vrn

Bay_HETPatn1_VRN.jpg

LP/-, PATN1/-, Vrn/Vrn

It appears PATN1's default state is a Blanket over the hips, and LP Promoter helps it spread across the body

Removing LP Promoter Genes While Adding Darkening Genes

Bay_HOMPatn1_lpp1_lpp2_VRN_STY.jpg

LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn, Sty/Sty

Bay_HOMPatn1_VRN_STY.jpg

LP/-, PATN1/PATN1, Vrn/Vrn, Sty/Sty

  

Bay_HOMPatn1_lpp2_VRN_STY_DP.jpg

LP/-, PATN1/PATN1, LPP2/-, Vrn/Vrn, Sty/Sty, DP/DP

Bay_HOMPatn1_lpp1_VRN_STY_DP.jpg

LP/-, PATN1/PATN1, LPP1/-, Vrn/Vrn, Sty/Sty, DP/DP

Final Observations

  1. If LPP2 is present, Spot Size remains smaller regardless of LPP1 or not.
  2. Each time LPP2 was present with Homozygous PATN1, the result was a true Leopard Phenotype (i.g., visual appearance). 
  3. LPP2 Spreads Blanket Markings further than LPP1, in the presence of PATN1. 
  4. LPP1 Produces larger Spots than LPP2. 
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