# All About Appaloosas (WIP)

# Intro to Appaloosa Genetics

<p class="callout info">*This article will introduce you to the building blocks of Appaloosa Genetics for Hunt&amp;Jump. After you have finished reading, you should be able to start your own program!*</p>

#### **Proposed Talking Points**

1. In Game Vocabulary
2. The Appaloosa Spotting Genes
3. Hidden Interactions in Appaloosa Spotting
4. Genes Independent of Appaloosa Spotting

##### **In Game Vocabulary**

<details id="bkmrk-in-game-vocabulary-a"><summary>In Game Vocabulary</summary>

**Allele**: It is a variant, or mutation, to a gene (e.g., LPP1 and LPP2 are Alleles of the LP Promoter Gene). Rarely used in H&amp;J language, but seen in this article.

**Appaloosa Characteristics**: Appaloosa (LP) will add mottling to the horses skin as well as *Varnishing*. Also see *Bronzing* in this article.

**Gene**: A section of DNA that codes for a specific inheritance (e.g., Appaloosa is the Gene). For the purpose of H&amp;J, Alleles are called Genes. Genes are what give our horses a variety of colors and patterns.

**Gene Modification Treatment**: An in-game service that allows you to see and modify most of your Horses Genes.

**Heterozygous**: One copy of an Allele (i.e., LP/-). [See this section for more information](https://wiki.huntandjump.com/books/genetics-101/page/how-to-read-genetic-testing-results-a-complete-guide-to-understanding-your-horses-color).

**Homozygous**: Two copies of the same Allele (i.e., LP/LP). [See this section for more information](https://wiki.huntandjump.com/books/genetics-101/page/how-to-read-genetic-testing-results-a-complete-guide-to-understanding-your-horses-color).

**Incomplete Dominant**: Two copies of a Gene will have greater expression than one copy of a Gene.

**Pigment Density**: How concentrated (i.g., dark) a horses color is. Black is more dense than Bay, and Bay is more dense than Chestnut.

**Recessive**: Will only show the effect when two copies (i.e., Homozygous) of the Allele are present.

**Varnishing**: A Different Pattern from Roan or Gray, this Gene progressively lightens a horses color by the addition of White hairs throughout the coat.

</details>##### **The Appaloosa Spotting Genes**

The Appaloosa Spotting Genes are probably the most dynamic genes in all of Hunt &amp; Jump, which means they are also the most complicated to understand. I've been breeding them for years, and still find new things to learn about. Generally speaking your Appaloosa Spotting Genes are going to be influenced by three primary factors:

1. Other Appaloosa Spotting Genes
2. Pigment Density
3. Age

<table border="1" id="bkmrk-chestnut-lp%2F-%2C-patn1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">*[![HET_lp_patn1_HOMVRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homvrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homvrn.jpg)*

*Chestnut LP/-, PATN1/-, Vrn/Vrn*

</td><td class="align-center" style="width: 49.9383%;">*[![HET_lp_patn1_lpp1_HOMVRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-lpp1-homvrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-lpp1-homvrn.jpg)*

*Chestnut LP/-, PATN1/-, LPP1/-, Vrn/Vrn*

*(the addition of the LPP1 Allele increased Blanket size)*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">*[![NoVarnish_MultiDilute_14yo_NoDilutions.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/novarnish-multidilute-14yo-nodilutions.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/novarnish-multidilute-14yo-nodilutions.jpg)*

*Liver Chestnut LP/-, PATN1/-*

</td><td class="align-center" style="width: 49.9383%;">*[![NoVarnish_MultiDilute_14yo_DMUCR.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/novarnish-multidilute-14yo-dmucr.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/novarnish-multidilute-14yo-dmucr.jpg)*

*Liver Chestnut LP/-, PATN1/-, Cr/-, D/-, mu/-*

*(the addition of Dilution genes reduces Pigment and increased Varnishing from LP)*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![HET_lp_patn1_vrn_lpp2_dp_HOMsty_young.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-vrn-lpp2-dp-homsty-young.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-vrn-lpp2-dp-homsty-young.jpg)

</td><td class="align-center" style="width: 49.9383%;">[![HET_lp_patn1_vrn_lpp2_dp_HOMsty_23yo.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-vrn-lpp2-dp-homsty-23yo.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-vrn-lpp2-dp-homsty-23yo.jpg)

*The same horse on the left, but at age 23*

</td></tr></tbody></table>

<details id="bkmrk-the-appaloosa-spotti-1"><summary>The Appaloosa Spotting Genes</summary>

<table border="1" style="border-collapse: collapse; width: 100%; height: 2046.5px;"><tbody><tr><td style="width: 50.0642%;">**[![Appaloosa_LP.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/appaloosa-lp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/appaloosa-lp.jpg)**

*Black LP/-, Vrn/-*

</td><td style="width: 50.0642%;">[![HET_lp_patn1_HOMVrn_young.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homvrn-young.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homvrn-young.jpg)

*Bay Dun LP/-, PATN1/-, Vrn/Vrn*

</td></tr><tr style="height: 192.125px;"><td style="width: 50.0642%; height: 192.125px;">**Appaloosa (LP)**  
Heterozygous LP allows Appaloosa Characteristics to be visible on the horse. While Homozygous LP does not increase the expression of Appaloosa Characteristics, but may increase the expression of additional Appaloosa Spotting Genes.

</td><td style="width: 50.0642%; height: 192.125px;">**Appaloosa Blanket Size (PATN1)**  
This is the primary gene for controlling a horses Blanket size. PATN1 is an Incomplete Dominant gene and can be influenced by additional genes. Must have at least one copy of LP to be shown.

</td></tr><tr><td style="width: 50.0642%;">**[![Snowflake.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hfasnowflake.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hfasnowflake.jpg)**

*Bay LP/-, snow/snow*

</td><td style="width: 50.0642%;">**[![Halo01.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/halo01.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/halo01.jpg)**

*Brown Dun LP/-, PATN/-, LPP1/-, ASR1/-, H/H*

</td></tr><tr style="height: 214.516px;"><td style="width: 50.0642%; height: 214.516px;">**Snowflake Varnish (snow)**  
This gene alters normal Varnishing into white speckles, which grow in size and quantity over the horses lifetime. Snowflake Varnish is Recessive, and requires two copies (i.g., Homozygous) to be expressed. Must have at least one copy of LP to be shown, and may be affected by other genes. This gene will not affect Spots on the horses coats.

</td><td style="width: 50.0642%; height: 214.516px;">**Varnish Regulator 2 (H)**  
Also known as Halo Spots or Peacock Spots, this gene causes a Varnished ring around Spots. The act of Haloing can make the Spot appear smaller. This gene is Incomplete Dominant, and its expression will strengthen over a horses life time. Must have one copy of LP to be shown, and can be influenced by other genes. Two copies of LP will remove Spots.

</td></tr><tr><td style="width: 50.0642%;">**[![HET_lp_patn1_HOMSty_ASR1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homsty-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homsty-asr1.jpg)**

*Chestnut LP/-, PATN1/-, LPP1/LPP1, ASR1/ASR1*

</td><td style="width: 50.0642%;">*[![HET_lp_lpp1_lpp2_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-lpp1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-lpp1-lpp2-vrn.jpg)*

*Bay LP/-, LPP1/LPP2, Vrn/-*

</td></tr><tr style="height: 192.125px;"><td style="width: 50.0642%; height: 192.125px;">**Appaloosa Spot Regulator (ASR1)**  
This Incomplete Dominate Gene modifies Spot Size, and can be influenced by other genes. Must have one copy of LP to be shown. Two copies of LP will remove Spots.

</td><td style="width: 50.0642%; height: 192.125px;">**Varnish Promoter (Vrn)**   
The presence of this gene will increase Appaloosa Varnishing. This gene is Incomplete Dominant, and its expression will strengthen over a horses life time. It can also be influenced by other genes. Must have at least one copy of LP to be shown. This gene will not affect Spots on the horses coats.

</td></tr><tr><td style="width: 50.0642%;">[![HET_lp_patn1_HOMLPP102.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homlpp102.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homlpp102.jpg)

***Homozygous LPP1***

*Bay Dun LP/-, PATN1/-, LPP1/-*

</td><td style="width: 50.0642%;">[![HET_lp_patn1_HETlpp2_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-hetlpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-hetlpp2-vrn.jpg)

***Heterozygous LPP2***

*Bay Dun LP/-, PATN1/-, LPP2/-, Vrn/-*

</td></tr><tr><td style="width: 50.0642%;">**LP Promoter** This Incomplete Dominate Gene modifies Blanket and Spot Size. The two Alleles in this gene, LPP1 and LPP2 can work together or independently of PATN1 to produce a wide range of Blanket sizes.

</td><td style="width: 50.0642%;">**LPP1** - Extends the PATN1 Blanket, but Spot size remains the same.

 **LPP2** - Blanket size typically spreads further with LPP2, and Spot size decreases.

<p class="callout info">[*View this Study for more examples with PATN1.* ](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2)</p>

</td></tr></tbody></table>

</details>**Hidden Interactions in Appaloosa Spotting**

<details id="bkmrk-hidden-interactions--0"><summary>Hidden Interactions in Appaloosa Spotting</summary>

**Unknown Hidden Appaloosa Blanket Size Gene**  
When searching for horses, under Appaloosa Blanket Size you can select "must not have" for both options (- and PATN1). If no Pattern Genes are present, horses should technically be solid or near solid looking.

[![Search.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/search.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/search.png)

According to players on the Bluegrass Forum, this hidden gene is called PATN2, but I have seen mixed reports on what it looks like.

<p class="callout warning">Need clarification from Ammit about what is or isn't going on</p>

**When Using Gene Modification Treatment (GMT)** By viewing your horse in the Gene Modification Treatment Lab, you will most likely see the majority of their genes. However there are some genes that are hidden and unknown to us. One way to verify that our horses have something extra is by checking to see if we can alter the Gene. Below are the messages you may receive when checking to see if you can alter the PATN1 gene.

<table border="1" style="border-collapse: collapse; width: 100%; height: 636.157px;"><tbody><tr style="height: 151.391px;"><td style="width: 99.8718%; height: 151.391px;">[![GMT_PATN1.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/gmt-patn1.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/gmt-patn1.png)

</td></tr><tr style="height: 80.1719px;"><td style="width: 99.8718%; height: 80.1719px;">In this example the horse is PATN1/-. First we check to see if we can alter the PATN1 Allele, and are given the option to change it to the - Allele. This means the only two options for Alleles here is PATN1 or nothing.

</td></tr><tr style="height: 145px;"><td style="width: 99.8718%; height: 145px;">[![GMT_noPATN.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/gmt-nopatn.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/gmt-nopatn.png)</td></tr><tr style="height: 29.7969px;"><td style="width: 99.8718%; height: 29.7969px;">By checking the - Allele, we see we have the option to change it to PATN1. This means we could potentially make our horse one of the following combinations:

1. PANT1/-
2. PATN1/PATN1
3. -/-

</td></tr><tr style="height: 200px;"><td style="width: 99.8718%; height: 200px;">[![GMT_PATN2.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/gmt-patn2.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/gmt-patn2.png)</td></tr><tr style="height: 29.7969px;"><td style="width: 99.8718%; height: 29.7969px;">However, sometimes the - Allele is actually a Hidden Gene, and we can't directly test for it. But by checking in the GMT lab we can see if something is there when this message pops up. So here I have a horse with the - Allele, but it's not the same - Allele as before. It's a secret shhhh. </td></tr></tbody></table>

**Appaloosa Bronzing** Appaloosa Bronzing is considered an Appaloosa Characteristic, but has no known genetic control. Instead it is influenced by Pigment Density. Bronzing lightens the mane and tail, the lighter the horse, the greater the Bronzing. There are other genes that may mimic Bronzing, such as Cream, Dun, and Roan.

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td style="width: 99.8718%;">[![Bronz_lp_patn1_lpp2_noVrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bronz-lp-patn1-lpp2-novrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bronz-lp-patn1-lpp2-novrn.jpg)

*Bay LP/-, PATN1/-, LPP2/- (No Darkening Genes or Varnish Promoter)*

</td></tr></tbody></table>

<table border="1" class="align-center" style="border-collapse: collapse; width: 52.1795%; height: 91.5469px; background-color: #bfedd2; border-color: #BFEDD2;"><tbody><tr style="height: 61.75px;"><td style="width: 37.6878%; height: 61.75px;">**Dense Pigment**

(e.g., Black &amp; Brown)

</td><td style="width: 21.4255%;"></td><td style="width: 40.8867%; height: 61.75px;">**Less Dense Pigment**

(e.g., Bay &amp; Chestnut)

</td></tr><tr style="height: 29.7969px;"><td style="width: 37.6878%; height: 29.7969px;">No Bronzing</td><td style="width: 21.4255%;">----------&gt;</td><td style="width: 40.8867%; height: 29.7969px;">More Bronzing</td></tr></tbody></table>

</details>##### **Genes Independent of Appaloosa Spotting**

<details id="bkmrk-genes-independent-of-0"><summary>Genes Independent of Appaloosa Spotting</summary>

The following genes do not affect Appaloosa Spotting Genes, but you may see them on Appaloosas depending on other genes they carry.

Kit Genes  
Splash Genes  
Frame  
Phantom Genes  
White Factor

</details><p class="callout info">*This information was compiled by Cheshire Farms on the Bluegrass Server and is current as of November 26, 2023.   
Special thank you to Ammit and Cheshire Farms for contributing photos to this article.* </p>

# How Do Appaloosa Genes Work Together

<p class="callout info">*Appaloosa Spotting Genes are some of the most complex Genetics in Hunt&amp;Jump, they work together and react to one another in such a way, that every horse is unique.* </p>

#### **Proposed Talking Points**

1. In Game Vocabulary
2. How Do Appaloosa Genes Work Together

##### **In Game Vocabulary**

<details id="bkmrk-in-game-vocabulary-a"><summary>In Game Vocabulary</summary>

**Allele**: It is a variant, or mutation, to a gene (e.g., LPP1 and LPP2 are Alleles of the LP Promoter Gene). Rarely used in H&amp;J language, but seen in this article.

**Appaloosa Characteristics**: Appaloosa (LP) will add mottling to the horses skin as well as *Varnishing*.

**Gene**: A section of DNA that codes for a specific inheritance (e.g., Appaloosa is the Gene). For the purpose of H&amp;J, Alleles are called Genes. Genes are what give our horses a variety of colors and patterns.

**Heterozygous**: One copy of an Allele (i.e., LP/-). [See this section for more information](https://wiki.huntandjump.com/books/genetics-101/page/how-to-read-genetic-testing-results-a-complete-guide-to-understanding-your-horses-color).

**Homozygous**: Two copies of the same Allele (i.e., LP/LP). [See this section for more information](https://wiki.huntandjump.com/books/genetics-101/page/how-to-read-genetic-testing-results-a-complete-guide-to-understanding-your-horses-color).

</details>##### **How Do Appaloosa Genes Work Together**

**Heterozygous Appaloosa (LP) vs. Homozygous Appaloosa (LP/LP)** By itself, the LP gene is just a switch to turn on the expression of other genes. It will add Appaloosa Characteristics, but those characteristics do not change between Heterozygous and Homozygous expression. However when the LP gene is Homozygous, and paired with other Appaloosa Spotting Genes, you will see an extreme difference from the Heterozygous expression.

<table border="1" class="align-center" id="bkmrk-%5Bpicture-coming-soon" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9359%;">[![HET_lp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp.jpg)

*Bay LP/-*

</td><td class="align-center" style="width: 49.9359%;">[![HOM_LP_hetlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-lp-hetlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-lp-hetlpp2.jpg)

*Chestnut LP/LP, LPP2/-*

</td></tr><tr><td class="align-center" style="width: 49.9359%;">[![HET_lp_patn1_lpp1_HOM_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-lpp1-hom-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-lpp1-hom-asr1.jpg)

*Brown LP/-, PATN1/-, LPP1/-, ASR1/ASR1*

</td><td class="align-center" style="width: 49.9359%;">*[![HOM_LP_het_patn1_lpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-lp-het-patn1-lpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-lp-het-patn1-lpp1.jpg)*

*Liver Chestnut LP/LP, PATN1/-, LPP1/-, ASR1/ASR1*

</td></tr></tbody></table>

<p class="callout danger">Homozygous LP will remove Spots. Any genes that affect Spots will not be visible. </p>

The following sections describe some of the interactions within the Appaloosa Spotting Gene group. Expand each section to see examples.

<details id="bkmrk-heterozygous-%28lp%29-%2B-"><summary>Heterozygous (LP) + Appaloosa Blanket Size (PATN1)</summary>

**Heterozygous (LP) + Appaloosa Blanket Size (PATN1)**

LP/-, PATN1/- : smaller spotted blanket, usually over the hips  
LP/-, PATN1/PANT1 : larger spotted blanket, typically extending past the withers

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9359%;">[![HET_lp_HET_partn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-het-partn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-het-partn1.jpg)

*E/E, a/a, LP/-, PATN1/-*

</td><td class="align-center" style="width: 49.9359%;">[![HET_lp_HOM_partn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-hom-partn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-hom-partn1.jpg)

*E/E, a/a, LP/-, PATN1/PATN1*

</td></tr></tbody></table>

</details><details id="bkmrk-homozygous-%C2%A0%28lp%2Flp%29-"><summary>Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1)</summary>

**Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1)**

<p class="callout danger">Homozygous LP does not change Blanket size. It does however remove Spots from the Blanket. </p>

LP/LP, PATN1/- : smaller blanket, usually over the hips  
LP/LP, PATN1/PANT1 : larger blanket, typically extending past the withers

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9359%;">[![HOM_lp_HET_partn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-lp-het-partn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-lp-het-partn1.jpg)

*E/E, A/a, LP/LP, PATN1/-*

</td><td class="align-center" style="width: 49.9359%;">[![HOM_lp_HOM_partn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-lp-hom-partn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-lp-hom-partn1.jpg)

*E/E, A/a, LP/LP, PATN1/PATN1*

</td></tr></tbody></table>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--0"><summary>LP + Snowflake Varnish (snow)</summary>

**Heterozygous (LP) + Snowflake Varnish (snow)**

LP/-, snow/- : snowflake varnish will not show unless Homozygous  
LP/-, snow/snow : white spots that multiple with time, can be influenced by other genes

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 100%;">[![HET_lp_HOM_snow.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-hom-snow.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-hom-snow.jpg)

*E/E, A/a, LP/-, snow/snow*

</td></tr></tbody></table>

**Homozygous (LP/LP) + Snowflake Varnish (snow)**

<p class="callout danger">Homozygous LP does not change the expression of Snowflake. Will appear the same as Heterozygous LP. </p>

<p class="callout info">*Future Study on Snowflake interactions*</p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--1"><summary>LP + Varnish Regulator 2 (H)</summary>

**Heterozygous (LP) + Varnish Regulator 2 (H)**

LP/-, H/- : will have smaller expression of Halo ring  
LP/-, H/H: results in greater expression of Halo ring

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 50.0642%;">[![HETpatn1_vrn_lpp1_HOMsty_h.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetpatn1-vrn-lpp1-homsty-h.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetpatn1-vrn-lpp1-homsty-h.jpg)

*LP/-, PATN1/-1, LPP1/-, Vrn/-, Sty/Sty, H/-*

</td><td class="align-center" style="width: 50.0642%;">[![HETpatn1_vrn_lpp1_HOMsty_hh.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetpatn1-vrn-lpp1-homsty-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetpatn1-vrn-lpp1-homsty-hh.jpg)

*LP/-, PATN1/-1, LPP1/-, Vrn/-, Sty/Sty, H/H*

</td></tr></tbody></table>

 **Homozygous (LP/LP) + Varnish Regulator 2 (H)** <p class="callout danger">Since Homozygous LP removes Spots, the Varnish Regulator 2 Gene will have nothing to affect. </p>

<p class="callout info">*See [<span style="text-decoration: underline;">Study #2: Appaloosa + Halo</span>](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-2-appaloosa-halo) for more results on how Halo interacts with Appaloosa Spotting Genes and Base Color.*</p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--2"><summary>LP + Appaloosa Spot Regulator (ASR1)</summary>

**Heterozygous (LP) + Appaloosa Spot Regulator (ASR1)**

The nature of the Appaloosa Gene (LP) does not actually add spots to the coat. Because of this, without any spots to affect, ASR1 will not show in the presence of only LP.

<p class="callout info">See the section *LP + Appaloosa Blanket Size (PATN1) + Appaloosa Spot Regulator (ASR1)* below for more information.</p>

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 100%;">[![16744716.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/16744716.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/16744716.jpg)

*E/e, A/A, D/-. DFP1/DFP1, Sty/Sty, P/P, LP/-, ASR1/ASR1*

</td></tr></tbody></table>

**Homozygous (LP/LP) + Appaloosa Spot Regulator (ASR1)**

<p class="callout danger">Since Homozygous LP removes Spots, the Appaloosa Spot Regulator Gene will have nothing to affect regardless of what other Spotting Genes are present. </p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--3"><summary>LP + Varnish Promoter (Vrn)</summary>

**Heterozygous (LP) + Varnish Promoter (Vrn)**

LP/-, Vrn/- : single copy of Varnish speeds up Varnishing as the horse ages  
LP/-, Vrn/Vrn : two copies of Varnish makes a horse Varnish faster

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 50.0642%;">[![SmokyBlack_Varnish01.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/smokyblack-varnish01.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/smokyblack-varnish01.jpg)

*E/E, a/a, Cr/-, Sty/Sty, DP/-, LP/-, Vrn/-*

</td><td class="align-center" style="width: 50.0642%;">[![SmokyBlack_Varnish02.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/smokyblack-varnish02.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/smokyblack-varnish02.jpg) *E/E, a/a, Cr/-, Sty/Sty, DP/-, LP/-, Vrn/Vrn*

</td></tr><tr><td class="align-center" style="width: 50.0642%;">[![Brown_HET Vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/brown-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/brown-het-vrn.jpg)

*E/e, A/a, Sty/Sty, DP/DP, LP/-, Vrn/- , age 19*

</td><td class="align-center" style="width: 50.0642%;">[![Brown_HOM Vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/brown-hom-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/brown-hom-vrn.jpg)

*E/e, A/a, Sty/Sty, DP/DP, LP/-, Vrn/Vrn , age 19*

</td></tr></tbody></table>

**Homozygous (LP/LP) + Varnish Promoter (Vrn)**

<p class="callout danger">Homozygous LP does not change the expression of Varnish. Will appear the same as Heterozygous LP. </p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--4"><summary>Heterozygous (LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP1)</summary>

<p class="callout info">Check out *Heterozygous (LP) + Appaloosa Blanket Size (PATN1)* to see examples of PATN1 Blanket Size. LPP1 adds to PATN1 by spreading the Blanket. </p>

**Heterozygous (LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP1)**

LP/-, PATN1/-, LPP1/- : extends Blanket Size, generally over the ribs  
LP/-, PATN1/-, LPP1/LPP1 : extends Blanket Size, generally beyond the withers  
LP/-, PATN1/PATN1, LPP1/- : extends Blanket Size to Near-Leopard, typically leaves colored Points  
LP/-, PATN1/PATN1, LPP1/LPP1 : extends Blanket Size to Near-Leopard, typically leaves colored Points

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%; height: 931.125px;"><tbody><tr style="height: 436.672px;"><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HETappy_HETlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetappy-hetlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetappy-hetlpp1.jpg)

*E/e, A/a, LP/-, PATN1/-, LPP1/-*

</td><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HETappy_HOMlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetappy-homlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetappy-homlpp1.jpg)

*E/e, A/a, LP/-, PATN1/-, LPP1/LPP1*

</td></tr><tr style="height: 436.672px;"><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HETappy_HETlpp1_HOMPatn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetappy-hetlpp1-hompatn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetappy-hetlpp1-hompatn1.jpg)

*E/e, A/a, LP/-, PATN1/PATN1, LPP1/-*

</td><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HETappy_HOMlpp1_HOMPatn1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetappy-homlpp1-hompatn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetappy-homlpp1-hompatn1.jpg)

*E/e, A/a, LP/-, PATN1/PATN1, LPP1/LPP1*

</td></tr></tbody></table>

<p class="callout info">See [*Study #1: PATN1 + LPP1/LPP2*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2) to see more examples of LP Promoter interaction with Appaloosa Blanket Size</p>

</details><details id="bkmrk-homozygous-%28lp%2Flp%29-%2B"><summary>Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP1)</summary>

<p class="callout info">Check out *Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1)* to see examples of PATN1 Blanket Size. LPP1 adds to PATN1 by spreading the Blanket. </p>

<p class="callout danger">Homozygous LP does not change Blanket size. It does however remove Spots from the Blanket. </p>

**Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP1)**

LP/LP, PATN1/-, LPP1/- : extends Blanket Size, generally over the ribs  
LP/LP, PATN1/-, LPP1/LPP1 : extends Blanket Size, generally beyond the withers  
LP/LP, PATN1/PATN1, LPP1/- : extends Blanket Size to Near-Leopard, typically leaves colored Points  
LP/LP, PATN1/PATN1, LPP1/LPP1 : extends Blanket Size to Near-Leopard, typically leaves colored Points

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%; height: 931.125px;"><tbody><tr style="height: 436.672px;"><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HOMlp_HETpatn1_HETlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hetpatn1-hetlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hetpatn1-hetlpp1.jpg)

*E/e, a/a, LP/LP, PATN1/-, LPP1/-*

</td><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HOMlp_HETpatn1_HOMlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hetpatn1-homlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hetpatn1-homlpp1.jpg)

*E/e, a/a, LP/LP, PATN1/-, LPP1/LPP1*

</td></tr><tr style="height: 436.672px;"><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HOMlp_HOMpatn1_HETlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hompatn1-hetlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hompatn1-hetlpp1.jpg)

*E/e, a/a, LP/LP, PATN1/PATN1, LPP1/-*

</td><td class="align-center" style="width: 50.0642%; height: 436.672px;">[![HOMlp_HOMpatn1_HOMlpp1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hompatn1-homlpp1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hompatn1-homlpp1.jpg)

*E/e, a/a, LP/LP, PATN1/PATN1, LPP1/LPP1*

</td></tr></tbody></table>

<p class="callout info">See [*Study #1: PATN1 + LPP1/LPP2*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2) to see more examples of LP Promoter interaction with Appaloosa Blanket Size</p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--5"><summary>Heterozygous (LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP2)</summary>

<p class="callout info">Check out *Heterozygous (LP) + Appaloosa Blanket Size (PATN1)* to see examples of PATN1 Blanket Size. LPP1 adds to PATN1 by spreading the Blanket. </p>

**Heterozygous (LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP2)**

LP/-, PATN1/-, LPP2/- : extends Blanket Size beyond the withers, reduces Spot Size  
LP/-, PATN1/-, LPP2/LPP2 : extends Blanket Size up the neck and to the knees, extremely reduces Spot Size  
LP/-, PATN1/PATN1, LPP2/- : extends Blanket Size to a Near-Leopard, reduces Spot Size  
LP/-, PATN1/PATN1, LPP2/LPP2 : extends Blanket Size to a Leopard, extremely reduces Spot Size

<table border="1" style="border-collapse: collapse; width: 100%; height: 602.109px;"><tbody><tr style="height: 312.25px;"><td class="align-center" style="width: 49.9359%; height: 312.25px;">*[![HETlp_HETpatn1_HETlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetlp-hetpatn1-hetlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetlp-hetpatn1-hetlpp2.jpg)*

*E/e, A/a, Sty/-, P/P, LP/-, PATN1/-, LPP2/-*

</td><td class="align-center" style="width: 49.9359%; height: 312.25px;">*[![HETlp_HETpatn1_HOMlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetlp-hetpatn1-homlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetlp-hetpatn1-homlpp2.jpg)*

*E/e, A/a, Sty/-, P/P, LP/-, PATN1/-, LPP2/LPP2*

</td></tr><tr style="height: 289.859px;"><td class="align-center" style="width: 49.9359%; height: 289.859px;">*[![HETlp_HOMpatn1_HETlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetlp-hompatn1-hetlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetlp-hompatn1-hetlpp2.jpg)*

*E/e, A/a, Sty/-, P/P, LP/-, PATN1/PATN1, LPP2/-*

</td><td class="align-center" style="width: 49.9359%; height: 289.859px;">*[![HETlp_HOMpatn1_HOMlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hetlp-hompatn1-homlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hetlp-hompatn1-homlpp2.jpg)*

*E/e, A/a, Sty/-, P/P, LP/-, PATN1/PATN1, LPP2/LPP2*

</td></tr></tbody></table>

<p class="callout info">See [*Study #1: PATN1 + LPP1/LPP2*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2) to see more examples of LP Promoter interaction with Appaloosa Blanket Size</p>

</details><details id="bkmrk-homozygous-%28lp%2Flp%29-%2B-0"><summary>Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP2)</summary>

<p class="callout info">Check out *Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1)* to see examples of PATN1 Blanket Size. LPP1 adds to PATN1 by spreading the Blanket. </p>

<p class="callout danger">Homozygous LP does not change Blanket size. It does however remove Spots from the Blanket. </p>

**Homozygous (LP/LP) + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP2)**

LP/LP, PATN1/-, LPP2/- : extends Blanket Size, generally beyond the withers  
LP/LP, PATN1/-, LPP2/LPP2 : extends Blanket Size, generally up the neck and to the knees  
LP/LP, PATN1/PATN1, LPP2/- : extends Blanket Size to a Few Spot Pattern, typically leaves small colored Points  
LP/LP, PATN1/PATN1, LPP2/LPP2 : extends Blanket Size to a Few Spot Pattern, typically leaves horse White

<table border="1" style="border-collapse: collapse; width: 100%; height: 602.109px;"><tbody><tr style="height: 312.25px;"><td class="align-center" style="width: 49.9359%; height: 312.25px;">[![HOMlp_HETpatn1_HETlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hetpatn1-hetlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hetpatn1-hetlpp2.jpg)

*E/e, A/a, Sty/-, P/P, LP/LP, PATN1/-, LPP2/-*

</td><td class="align-center" style="width: 49.9359%; height: 312.25px;">[![HOMlp_HETpatn1_HOMlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hetpatn1-homlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hetpatn1-homlpp2.jpg)

*E/e, A/a, Sty/-, P/P, LP/LP, PATN1/-, LPP2/LPP2*

</td></tr><tr style="height: 289.859px;"><td class="align-center" style="width: 49.9359%; height: 289.859px;">[![HOMlp_HOMpatn1_HETlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hompatn1-hetlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hompatn1-hetlpp2.jpg)

*E/e, A/a, Sty/-, P/P, LP/LP, PATN1/PATN1, LPP2/-*

</td><td class="align-center" style="width: 49.9359%; height: 289.859px;">[![HOMlp_HOMpatn1_HOMlpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homlp-hompatn1-homlpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homlp-hompatn1-homlpp2.jpg)

*E/e, A/a, Sty/-, P/P, LP/LP, PATN1/PATN1, LPP2/LPP2*

</td></tr></tbody></table>

<p class="callout info">See [*Study #1: PATN1 + LPP1/LPP2*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2) to see more examples of LP Promoter interaction with Appaloosa Blanket Size</p>

</details><details id="bkmrk-heterozygous-%28lp%29-%2B--6"><summary>LP + Appaloosa Blanket Size (PATN1) + LP Promoter (LPP1 &amp; LPP2)</summary>

<p class="callout danger">Homozygous LP does not change Blanket size. It does however remove Spots from the Blanket. </p>

**LP + LP Promoter (LPP1 &amp; LPP2)**

LP/-, LPP1/LPP2 : when combined the two LP Promoter genes create a small spotted blanket over the hips  
LP/-, PATN1/-, LPP1/LPP2 : creates a large spotted blanket with reduced Spot Size  
LP/LP, LPP1/LPP2 : when combined the two LP Promoter genes create a small blanket over the hips  
LP/LP, PATN1/-, LPP1/LPP2 : creates a large blanket

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%; height: 347.641px;"><tbody><tr style="height: 312.25px;"><td class="align-center" style="width: 50.0642%; height: 312.25px;">[![LP_LPP1_LPP2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/lp-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/lp-lpp1-lpp2.jpg)

*e/e, A/A, Sty/Sty, LP/-, LPP1/LPP2*

</td><td style="width: 50.0642%; height: 312.25px;">[![LP_PATN1_LPP1_LPP2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/lp-patn1-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/lp-patn1-lpp1-lpp2.jpg)

*e/e, A/A, Sty/Sty, LP/-, PATN1/-, LPP1/LPP2*

</td></tr><tr style="height: 35.3906px;"><td style="width: 50.0642%; height: 35.3906px;">[![LPhom_LPP1_LPP2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/lphom-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/lphom-lpp1-lpp2.jpg)

*e/e, A/A, Sty/Sty, LP/LP, LPP1/LPP2*

</td><td style="width: 50.0642%; height: 35.3906px;">[![LPhom_PATN1_LPP1_LPP2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/lphom-patn1-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/lphom-patn1-lpp1-lpp2.jpg)

*e/e, A/A, Sty/Sty, LP/LP, PATN1/-, LPP1/LPP2*

</td></tr></tbody></table>

<p class="callout info">See [*Study #1: PATN1 + LPP1/LPP2*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-1-patn1-lpp1lpp2) to see more examples of LP Promoter interaction with Appaloosa Blanket Size</p>

</details><details id="bkmrk-lp-%2B-appaloosa-blank"><summary>LP + Appaloosa Blanket Size (PATN1) + Appaloosa Spot Regulator (ASR1)</summary>

<p class="callout danger">Homozygous LP does not change Spot size. It does however remove Spots from the Blanket. </p>

**LP + Appaloosa Blanket Size (PATN1) + Appaloosa Spot Regulator (ASR1)**

LP/-, PATN1/-, ASR1/- : the presence of ASR1 will create larger spots  
LP/-, PATN1/-, ASR1/ASR1 : two copies of ASR1 will produce even larger spots  
LP/LP, PATN1/-, ASR1/ASR1 : homozygous LP removes Spots, ASR1 won't be visible

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9359%;">[![HET_patn1_HET_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-patn1-het-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-patn1-het-asr1.jpg)

*E/e, A/A, LP/-, PATN1/-, ASR1/-*

</td><td class="align-center" style="width: 49.9359%;">[![HET_patn1_HOM_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-patn1-hom-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-patn1-hom-asr1.jpg)

*E/e, A/A, LP/-, PATN1/-, ASR1/ASR1*

</td></tr><tr><td class="align-center" style="width: 49.9359%;">[![HOM_patn1_HET_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-patn1-het-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-patn1-het-asr1.jpg)

*E/e, A/A, LP/-, PATN1/PATN1, ASR1/-*

</td><td class="align-center" style="width: 49.9359%;">[![HOM_patn1_HOM_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-patn1-hom-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-patn1-hom-asr1.jpg)

*E/e, A/A, LP/-, PATN1/PATN1, ASR1/ASR1*

</td></tr></tbody></table>

<p class="callout info">Check out [*Study #3: Appaloosa Spot Regulator (ASR1)*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-3-appaloosa-spot-regulator-asr1) for more examples of how other genes react with ASR1. </p>

</details><details id="bkmrk-how-appaloosa-genes--0"><summary>Snowflake Varnish (snow) + Varnish Promoter (Vrn) </summary>

 **Snowflake Varnish (snow) + Varnish Promoter (Vrn)**

snow/snow, -/- : base color is clear, snowflake spotting is spread out  
snow/snow, Vrn/- : base color is varnished, snowflake spotting is more concentrated  
snow/snow, Vrn/Vrn : base color is extremely varnished, snowflake spotting is densely concentrated

<table border="1" class="align-center" style="border-collapse: collapse; width: 100%;"><tbody><tr><td style="width: 33.2892%;">[![HOMSnow_noVrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homsnow-novrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homsnow-novrn.jpg)

*Chestnut LP/-, snow/snow, -/-*

</td><td style="width: 33.2892%;">[![HOMSnow_HETVrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homsnow-hetvrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homsnow-hetvrn.jpg)

*Chestnut LP/-, snow/snow, Vrn/-*

</td><td style="width: 33.2933%;">[![HOMSnow_HOMVrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/homsnow-homvrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/homsnow-homvrn.jpg)

*Chestnut LP/-, snow/snow, Vrn/Vrn*

</td></tr></tbody></table>

</details><p class="callout info">*This information was compiled by Cheshire Farms on the Bluegrass Server and is current as of November 26, 2023.   
Special thank you to Ammit, Cavalynn and Cheshire Farms for contributing photos to this article.* </p>

# Interactions of Appaloosa Spotting with Other Genes

<p class="callout info">*Appaloosa Spotting Genes react with Non-Appaloosa Genetics, making them a little hard to predict and breed true. However this article is here to help you better understand how things like Pigment Density can produce larger spots.* </p>

#### **Proposed Talking Points**

1. In Game Vocabulary
2. How Do Appaloosa Genes Interact with Non-Appaloosa Genes
3. Interactions of Appaloosa Spotting and Gender

##### **In Game Vocabulary**

<details id="bkmrk-in-game-vocabulary-a"><summary>In Game Vocabulary</summary>

Base Color

Pigment Density

Pinto

Shade

</details>##### **How Do Appaloosa Genes Interact with Non-Appaloosa Genes**

<details id="bkmrk-appaloosa-spotting-%2B"><summary>Appaloosa Spotting + Black &amp; Red Pigment</summary>

**Appaloosa Spotting + Black &amp; Red Pigment**

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 33.2892%;">*[![HET_lp_patn1_HOMsty_HOMDP-01.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homsty-homdp-01.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homsty-homdp-01.jpg)*

*Black LP/-. PATN1/-, Sty/Sty, DP/DP*

</td><td class="align-center" style="width: 33.2892%;">*[![HET_lp_patn1-HOMSTY-02.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-homsty-02.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-homsty-02.jpg)*

*Bay LP/-. PATN1/-, Sty/Sty*

</td><td class="align-center" style="width: 33.2933%;">*[![HET_lp_patn1-03.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-lp-patn1-03.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-lp-patn1-03.jpg)*

*Chestnut LP/-. PATN1/-*

</td></tr></tbody></table>

Pigment density (i.g., how dark a color is), can alter the expression of Appaloosa Spotting Genes by changing the rate they Varnish, or modifying spot size. On a scale of most dense to least dense, we have Black being the most dense, then Bay, followed by Chestnut being the least dense. With the addition of Darkening Genes, we can modify spot size even on Black coats.

<table border="1" class="align-center" style="border-collapse: collapse; width: 42.0513%; height: 139px; background-color: #bfedd2;"><tbody><tr><td class="align-right" style="width: 26.8973%;">  
</td><td class="align-center" style="width: 48.0262%;">**Rate of Varnishing**</td><td class="align-center" style="width: 25.0765%;">**Spot Size**</td></tr><tr><td class="align-right" style="width: 26.8973%;">**Black**</td><td class="align-center" style="width: 48.0262%;">Slow</td><td class="align-center" style="width: 25.0765%;">Larger</td></tr><tr><td class="align-right" style="width: 26.8973%;">**Bay**</td><td class="align-center" style="width: 48.0262%;">Medium</td><td class="align-center" style="width: 25.0765%;">Average</td></tr><tr><td class="align-right" style="width: 26.8973%;">**Chestnut**</td><td class="align-center" style="width: 48.0262%;">Fast</td><td class="align-center" style="width: 25.0765%;">Smaller</td></tr></tbody></table>

<p class="callout info">*The chart above illustrates how the horses Base Color can influence Varnishing as well as Spot Size. It is important to note that Varnishing and Spot Size are independent of one another.* </p>

</details><details id="bkmrk-how-appaloosa-genes--0"><summary>Appaloosa Spotting + Darkening Genes (Sty, Sty+ and DP)</summary>

**Appaloosa Spotting + Darkening Genes**

Currently there are two genes that cause Pigment to darken, they are Sooty (Sty and S+) and Dense Pheomelanin (DP). These genes can work individually, or combine together to increase the range of Shade and Spot Size.

**Sooty (Sty and S+)**: Darkens a horses coat, and can also increase Spot Size, even in Black Based horses. Sooty + also darkens the coat, but breaks up the pigment to produce heavy dappling.

**Dense Pheomelanin (DP)**: Increases the production of Red Pigment, and can also increase Spot Size, even in Black Based horses.

<table border="0" class="align-center" style="border-collapse: collapse; width: 80%;"><tbody><tr><td style="width: 9.63082%;">  
</td><td class="align-center" style="width: 29.8555%;">[![02.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/02.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/02.png)</td><td class="align-center" style="width: 30.0176%;">[![01.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/01.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/01.png)</td><td class="align-center" style="width: 30.6566%;">[![03.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/03.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/03.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">  
</td><td class="align-center" style="width: 29.8555%;">[![Black.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay.png)</td><td class="align-center" style="width: 30.6566%;">[![Chestnut.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">[![04.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/04.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/04.png)</td><td class="align-center" style="width: 29.8555%;">[![Black_HETsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-hetsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-hetsty.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay_HETsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetsty.png)</td><td class="align-center" style="width: 30.6566%;">[![Chestnut_HETsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-hetsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-hetsty.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">[![07.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/07.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/07.png)</td><td class="align-center" style="width: 29.8555%;">[![Black_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-homdp.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-homdp.png)</td><td class="align-center" style="width: 30.6566%;">[![Chestnut_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-homdp.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">[![05.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/05.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/05.png)</td><td class="align-center" style="width: 29.8555%;">[![Black_HOMsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-homsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-homsty.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay_HOMsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-homsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-homsty.png)</td><td class="align-center" style="width: 30.6566%;">[![Chestnut_HOMsty.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-homsty.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-homsty.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">[![06.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/06.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/06.png)</td><td class="align-center" style="width: 29.8555%;">[![Black_HETsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-hetsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-hetsty-homdp.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay_HETsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetsty-homdp.png)</td><td class="align-center" style="width: 30.6566%;"> [![Chestnut_HETsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-hetsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-hetsty-homdp.png)</td></tr><tr><td class="align-right" style="width: 9.63082%;">[![08.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/08.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/08.png)</td><td class="align-center" style="width: 29.8555%;">[![Black_HOMsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-homsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-homsty-homdp.png)</td><td class="align-center" style="width: 30.0176%;">[![Bay_HOMsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-homsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-homsty-homdp.png)</td><td class="align-center" style="width: 30.6566%;">[![Chestnut_HOMsty_HOMdp.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-homsty-homdp.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-homsty-homdp.png)</td></tr></tbody></table>

<p class="callout info">*The chart above illustrates how the horses Base Color and Darkening Genes can influence Spot Size. It is important to note that these Genes work on Black Based horses as well, even if they are not visually seen.* </p>

**Sooty Plus (S+) and Spot Size**

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 33.2892%;">[![Black_SootyPlus.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-sootyplus.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-sootyplus.jpg)</td><td class="align-center" style="width: 33.2892%;">[![Bay_SootyPlus.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-sootyplus.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-sootyplus.jpg)</td><td class="align-center" style="width: 33.2933%;">[![Chestnut_SootyPlus.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-sootyplus.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-sootyplus.jpg)</td></tr></tbody></table>

All three horses above are LP/-, PATN1/-, S+/S+. While Sooty + changes the shade of a horse, it can also creates larger Spots.

</details><details id="bkmrk-appaloosa-spotting-%2B-0"><summary>Appaloosa Spotting + Pigment Density Regulator (Bl)</summary>

**Appaloosa Spotting + Pigment Density Regulator (Bl)**

Currently Pigment Density Regulator (Bl) does three different things in the game:

1. Reduces the amount of White from Pinto Related Genes
2. Adds freckling to White Pinto Related Patterns
3. Deepens the Red in the Base Color

Of these three things, the only one that affects Appaloosa Genes is the deepening of Red. It is a very minor change, unlike Sooty and Dense Pheomelanin.

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 33.3761%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)

Chestnut + No Bl

</td><td class="align-center" style="width: 33.3761%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-bl.jpg)

Chestnut + Bl/-

</td><td class="align-center" style="width: 33.3761%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)

Chestnut + Bl/Bl

</td></tr></tbody></table>

Pigment Density Regulator (Bl) changed the Shade of the Chestnut horse with each copy of Bl. Because it was Darkening the coat, the effect of Varnishing was also reduced (more Varnishing with no Bl).

<p class="callout info">Check out [*Study #4: Appaloosa Spotting + Pigment Density Regulator (Bl)*](https://wiki.huntandjump.com/books/all-about-appaloosas-wip/page/study-4-appaloosa-spotting-pigment-density-regulator-bl) to see more examples of Pigment Density Regulator and Darkening Genes.</p>

</details><details id="bkmrk-appaloosa-spotting-%2B-1"><summary>Appaloosa Spotting + Lightening Genes</summary>

**Appaloosa Spotting + Lightening Genes**

Cream/Pearl/Sun/Sno  
Dun/Non-Dun  
Champagne  
Silver  
Mushroom

</details><details id="bkmrk-appaloosa-spotting-%2B-2"><summary>Appaloosa Spotting + Pigment Removal</summary>

**Appaloosa Spotting + Pigment Removal**

Grey

</details><details id="bkmrk-appaloosa-spotting-%2B-3"><summary>Appaloosa Spotting + Other Genes</summary>

**Appaloosa Spotting + Other Genes**

Flaxen  
Pangare

</details>##### **Interactions of Appaloosa Spotting and Gender**  


<details id="bkmrk-interactions-of-appa-0"><summary>Interactions of Appaloosa Spotting and Gender</summary>

Appaloosa Varnishing will have different expressions between Mares and Stallions, with Mares varnishing a little faster than Stallions.

<table border="1" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9359%;">*[![blackstud_withVarnish.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/blackstud-withvarnish.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/blackstud-withvarnish.jpg)*

*Stallion showing less Varnishing*

</td><td class="align-center" style="width: 49.9359%;">[![blackmare_WithVarnish.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/blackmare-withvarnish.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/blackmare-withvarnish.jpg)

*Mare showing more Varnishing*

</td></tr><tr><td class="align-center" style="width: 49.9359%;">[![NoVarnish_MultiDilute_14yo_BrownStud.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/novarnish-multidilute-14yo-brownstud.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/novarnish-multidilute-14yo-brownstud.png)

*Stallion showing less Varnishing at 14 yo*

</td><td class="align-center" style="width: 49.9359%;">[![NoVarnish_MultiDilute_14yo_Brown.png](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/novarnish-multidilute-14yo-brown.png)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/novarnish-multidilute-14yo-brown.png)

*Mare showing more Varnishing at 14 yo*

</td></tr></tbody></table>

</details><p class="callout info">*This information was compiled by Cheshire Farms on the Bluegrass Server and is current as of November 26, 2023.   
Special thank you to Ammit and Cheshire Farms for contributing photos to this article.* </p>

# Interactions of Appaloosa Spotting with Fantasy Genes

<p class="callout info">*The following information has been pieced together from various sources, in hopes of helping the reader better understand H&amp;J's Appaloosa Spotting Genetics.* </p>

#### **Proposed Talking Points**

1. In Game Vocabulary
2. How Does Appaloosa Spotting Work with Fantasy Genes

##### **In Game Vocabulary**

<details id="bkmrk-in-game-vocabulary-a"><summary>In Game Vocabulary</summary>

 </details>##### **How Does Appaloosa Spotting Work with Fantasy Genes**

<details id="bkmrk-how-appaloosa-genes--0"><summary>How Does Appaloosa Spotting Work with Fantasy Genes</summary>

Chocolate, Platinum, and Chinchilla might interact with Appaloosa Genes because they modify pigment. But need to get clarification from Ammit on this.

</details><p class="callout info">*This information was compiled by Cheshire Farms on the Bluegrass Server and is current as of November 14, 2023.* </p>

# Study #1: PATN1 + LPP1/LPP2

<p class="callout info">This study looks at how LPP1 and LPP2 interact with PATN1 when Varnish Promoters are present, and Darkening Genes are added. </p>

**[![Bay_HOMPatn1_lpp1_lpp2_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp1-lpp2-vrn.jpg)  
Study #1: 8yo Bay Appaloosa Mare *LPP1/LPP2***  
*E/E, A/A, f/-, P/P, W20/-, LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn*

**Removing LP Promoter Genes**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2Fpatn1%2C-l" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp1_lpp2_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp1-lpp2-vrn.jpg)

*LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp2_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp2-vrn.jpg)

*LP/-, PATN1/PATN1, LPP2/-, Vrn/Vrn*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp1_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp1-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp1-vrn.jpg)

*LP/-, PATN1/PATN1, LPP1/-, Vrn/Vrn*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-vrn.jpg)

*LP/-, PATN1/PATN1, Vrn/Vrn*

</td></tr></tbody></table>

<p class="callout info">Notice how she went from Leopard to Near Leopard with the removal of LP Promoter Genes (LPP1 and LPP2)</p>

**Heterozygous for PATN1**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2F-%2C-lpp1%2F" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HETPatn1_lpp1_lpp2_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetpatn1-lpp1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetpatn1-lpp1-lpp2-vrn.jpg)

*LP/-, PATN1/-, LPP1/LPP2, Vrn/Vrn*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HETPatn1_lpp2_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetpatn1-lpp2-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetpatn1-lpp2-vrn.jpg)

*LP/-, PATN1/-, LPP2/-, Vrn/Vrn*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HETPatn1_lpp1_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetpatn1-lpp1-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetpatn1-lpp1-vrn.jpg)

*LP/-, PATN1/-, LPP1/-, Vrn/Vrn*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HETPatn1_VRN.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hetpatn1-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hetpatn1-vrn.jpg)

*LP/-, PATN1/-, Vrn/Vrn*

</td></tr></tbody></table>

<p class="callout info">It appears PATN1's default state is a Blanket over the hips, and LP Promoter helps it spread across the body</p>

**Removing LP Promoter Genes While Adding Darkening Genes**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2Fpatn1%2C-l-0" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp1_lpp2_VRN_STY.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp1-lpp2-vrn-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp1-lpp2-vrn-sty.jpg)

*LP/-, PATN1/PATN1, LPP1/LPP2, Vrn/Vrn, Sty/Sty*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_VRN_STY.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-vrn-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-vrn-sty.jpg)

*LP/-, PATN1/PATN1, Vrn/Vrn, Sty/Sty*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp2_VRN_STY_DP.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp2-vrn-sty-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp2-vrn-sty-dp.jpg)

*LP/-, PATN1/PATN1, LPP2/-, Vrn/Vrn, Sty/Sty, DP/DP*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_HOMPatn1_lpp1_VRN_STY_DP.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-hompatn1-lpp1-vrn-sty-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-hompatn1-lpp1-vrn-sty-dp.jpg)

*LP/-, PATN1/PATN1, LPP1/-, Vrn/Vrn, Sty/Sty, DP/DP*

</td></tr></tbody></table>

**Final Observations**

1. If LPP2 is present, Spot Size remains smaller regardless of LPP1 or not.
2. Each time LPP2 was present with Homozygous PATN1, the result was a true Leopard Phenotype (i.g., visual appearance).
3. LPP2 Spreads Blanket Markings further than LPP1, in the presence of PATN1.
4. LPP1 Produces larger Spots than LPP2.

# Study #2: Appaloosa + Halo

<p class="callout info">This study looks at how Appaloosas with Halo Spots react when their Base Colors change. </p>

**[![Black_lpp1_lpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp1-lpp2.jpg)  
Study #1: 8yo Black Appaloosa Mare**  
*E/e, a/a, LP/-, LPP1/LPP2*

**Adding Varnish Regulator 2 (H/H)**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2Fpatn1%2C-l" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">**[![Black_lpp1_lpp2.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp1-lpp2.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp1-lpp2.jpg)**

*Non-Halo*

</td><td class="align-center" style="width: 49.9383%;">[![Black_lpp1_lpp2_HH.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp1-lpp2-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp1-lpp2-hh.jpg)

*Black LP/-, LPP1/LPP2, H/H*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![Bay_lpp1_lpp2_HH.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-lpp1-lpp2-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-lpp1-lpp2-hh.jpg)

*Bay LP/-, LPP1/LPP2, H/H*

</td><td class="align-center" style="width: 49.9383%;">[![Chestnut_lpp1_lpp2_HH.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-lpp1-lpp2-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-lpp1-lpp2-hh.jpg)

*Chestnut LP/-, LPP1/LPP2, H/H*

</td></tr></tbody></table>

<p class="callout info">Chestnut Blanket is slightly larger than on Bay and Black</p>

**Removing LP Promoters &amp; Adding PATN1**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2F-%2C-lpp1%2F" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![Black_lpp1_HH.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp1-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp1-hh.jpg)

*Black LP/-, LPP1/-, H/H*

</td><td class="align-center" style="width: 49.9383%;">[![Black_lpp2_HH.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp2-hh.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp2-hh.jpg)

*Black LP/-, LPP2/-, H/H*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![Black_lpp1_HH_hetPATN1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp1-hh-hetpatn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp1-hh-hetpatn1.jpg)

*Black LP/-, PATN1/-, LPP1/-, H/H*

</td><td class="align-center" style="width: 49.9383%;">[![Black_lpp2_HH_hetPATN1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/black-lpp2-hh-hetpatn1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/black-lpp2-hh-hetpatn1.jpg)

*Black LP/-, PATN1/-, LPP2/-, H/H*

</td></tr></tbody></table>

<p class="callout info">Study #1 showed that in the presence of PATN1, LPP2 actually produced larger white Blanket markings than LPP1 did. But if PATN1 is removed, LPP2 leaves more white than LPP1 (see top row)</p>

**Adding Darkening Genes on Homozygous PATN1**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2Fpatn1%2C-l-0" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![Chestnut_lpp1_HH_HOMPATN1_HOMsty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnut-lpp1-hh-hompatn1-homsty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnut-lpp1-hh-hompatn1-homsty.jpg)

*Chestnut LP/-, PATN1/PATN1, LPP1/-, H/H, Sty/Sty*

</td><td class="align-center" style="width: 49.9383%;">[![Bay_lpp1_HH_HOMPATN1_HOMsty_HOMDP.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/bay-lpp1-hh-hompatn1-homsty-homdp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/bay-lpp1-hh-hompatn1-homsty-homdp.jpg)

*Bay LP/-, PATN1/PATN1, LPP1/-, H/H, Sty/Sty, DP/DP*

</td></tr></tbody></table>

<p class="callout info">Pigment density influenced Spot Size as well as Spot Spread. Halo rings are clearly seen with Homozygous Sooty or Homozygous Sooty and Dense Pheomelanin.</p>

**Final Observations**

1. Because Halo gives the impression of shrinking your Spots, it is best seen on horses with larger spots.
2. Halo doesn't appear to be as dynamic when changing the base color. Many photos weren't included because the spot size and shape looked the same as the one before it.
3. With LPP1 &amp; LPP2 you may be able to see Halo without PATN1. But either LPP1 or LPP2 by itself will not produce Halo marks.
4. Darkening genes like DP and Sty make Halo more visible.

# Study #3: Appaloosa Spot Regulator (ASR1)

<p class="callout info">This study looks at how ASR1 is influenced by other genes. </p>

**[![HOM_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1.jpg)  
Study #3: 1yo Bay Appaloosa**  
*E/e, A/A, LP/-, PATN1/-, ASR1/ASR1*

**Removing ASR1 Genes**

<table border="1" id="bkmrk-lp%2F-%2C-patn1%2Fpatn1%2C-l-0" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![HET_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-asr1.jpg)

*Bay LP/-, PATN1/-, ASR1/-*

</td><td class="align-center" style="width: 49.9383%;">[![No_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/no-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/no-asr1.jpg)

*Bay LP/-, PATN1/-*

</td></tr></tbody></table>

<p class="callout info">*ASR1 is an incomplete dominate gene, and will have a stronger effect on the spots when Homozygous*</p>

**Adding Sooty (Sty) + Removing ASR1 Genes**

<table border="1" id="bkmrk-bay-lp%2F-%2C-patn1%2F-%2C-a" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HET_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-het-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-het-sty.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1, Sty/-*

</td><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HOM_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-hom-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-hom-sty.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1, Sty/Sty*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![HET_asr1_HOM_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/het-asr1-hom-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/het-asr1-hom-sty.jpg)

*Bay LP/-, PATN1/-, ASR1/-, Sty/Sty*

</td><td class="align-center" style="width: 49.9383%;">[![No_asr1_HOM_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/no-asr1-hom-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/no-asr1-hom-sty.jpg)

*Bay LP/-, PATN1/-, Sty/Sty*

</td></tr></tbody></table>

<p class="callout info">*Notice how the addition of Sooty causes the spots to spread further apart. Referring back to the original horse, you can see their spots are very close together, even overlapping. When Sooty is added, the Spots spread further apart, giving the distinct impression of larger spots.* </p>

**Adding Dense Pheomelanin (DP) + Sooty (Sty)**

<table border="1" id="bkmrk-genetics-genetics-%C2%A0%C2%A0" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HET_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-het-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-het-dp.jpg)

  
*Bay LP/-, PATN1/-, ASR1/ASR1, DP/-*

</td><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HOM_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-hom-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-hom-dp.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1, DP/DP*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HOM_dp_HET_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-hom-dp-het-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-hom-dp-het-sty.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1, DP/DP, Sty/-*

</td><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_HOM_dp_HOM_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-hom-dp-hom-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-hom-dp-hom-sty.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1, DP/DP, Sty/Sty*

</td></tr></tbody></table>

<p class="callout info">*Darkening genes don't add to the Spot Size, but they do make the spots spread further apart.* </p>

**Adding Diluting Genes to Appaloosa Spot Regulator (ASR1)**

<table border="1" id="bkmrk-bay-lp%2F-%2C-patn1%2F-%2C-a-0" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">[![HOM_asr1.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1.jpg)

*Bay LP/-, PATN1/-, ASR1/ASR1*

</td><td class="align-center" style="width: 49.9383%;">[![HOM_asr1_cream.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/hom-asr1-cream.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/hom-asr1-cream.jpg)

*Palomino LP/-, PATN1/-, ASR1/ASR1*

</td></tr></tbody></table>

<p class="callout info">Reducing the pigment concentration through use of Dilution genes makes minute changes to the white pattern. In the case of Bay vs Palomino, the Palomino has lost some of the smaller freckled spots on their Blanket, giving the impression of larger white areas. </p>

**Final Observations**

1. In the presence of Homozygous ASR1, adding and removing pigment doesn't appear to change Spot Size. It does however influence Spot Spread.

# Study #4: Appaloosa Spotting + Pigment Density Regulator (Bl)

<p class="callout info">This study looks at how the Pigment Density Regulator (Bl) interacts with Appaloosa Spotting and different Base Colors. </p>

[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)

**Study #4: 9yo Chestnut Appaloosa** *(e/e, A/A, f/f, p/p, LP/-, PATN1/-, Vrn/-)*

**Chestnut + Pigment Density Regulator (Bl)**

<table border="1" id="bkmrk-no-darkening-genes-h" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 33.2909%;">*No Darkening Genes*</td><td class="align-center" style="width: 33.2909%;">*HET Sooty*</td><td class="align-center" style="width: 33.2948%;">*HOM Sooty*</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn.jpg)

No Bl

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty.jpg)

No Bl + Sty/-

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_sty.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty.jpg)

No Bl + Sty/Sty

</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-bl.jpg)

Bl/-

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_sty_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty-het-bl.jpg)

Bl/-, Sty/-

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_sty_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty-het-bl.jpg)

Bl/-, Sty/Sty

</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)

Bl/Bl

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_sty_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-sty-hom-bl.jpg)

Bl/Bl, Sty/-

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_sty_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-sty-hom-bl.jpg)

Bl/Bl, Sty/Sty

</td></tr></tbody></table>

<p class="callout info">Of the nine images above, all of them except the very last one (Bl/Bl, Sty/Sty) showed any shade changes. In this case, Bl/Bl, Sty/Sty was the same as Bl/-, Sty/Sty. Spot Size only changed with the addition of Sooty, it did not change with Bl. </p>

<table border="1" id="bkmrk-het-dp-hom-dp-sooty-" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 33.2909%;">*HET DP*</td><td class="align-center" style="width: 33.2909%;">*HOM DP*</td><td class="align-center" style="width: 33.2948%;">*Sooty + DP*</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp.jpg)

No Bl + DP/-

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp.jpg)

No Bl + DP/DP

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_PATN1_vrn_sty_HOM_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-patn1-vrn-sty-hom-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-patn1-vrn-sty-hom-dp.jpg)

No Bl + Sty/-, DP/DP

</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_dp_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp-het-bl.jpg)

Bl/-, DP/-

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_dp_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp-het-bl.jpg)

Bl/-, DP/DP

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_PATN1_vrn_HOM_sty_HOM_dp.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-patn1-vrn-hom-sty-hom-dp.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-patn1-vrn-hom-sty-hom-dp.jpg)

Bl/-, Sty/Sty, DP/DP

</td></tr><tr><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HET_dp_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-het-dp-hom-bl.jpg)

Bl/Bl, DP/-

</td><td class="align-center" style="width: 33.2909%;">[![ChestnutDraft_HET_lp_HET_PATN1_HET_vrn_HOM_dp_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-het-patn1-het-vrn-hom-dp-hom-bl.jpg)

Bl/Bl, DP/DP

</td><td class="align-center" style="width: 33.2948%;">[![ChestnutDraft_HET_lp_PATN1_vrn_HOM_sty_HOM_dp_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/chestnutdraft-het-lp-patn1-vrn-hom-sty-hom-dp-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/chestnutdraft-het-lp-patn1-vrn-hom-sty-hom-dp-hom-bl.jpg)

Bl/Bl, Sty/Sty, DP/DP

</td></tr></tbody></table>

<p class="callout info">This test saw less Shade variation, specially when the Darkening Genes were Homozygous. At some point the Pigment Density Regulator can only deepen the Base color so much. With Sty and DP were combined with Bl, there was no change in Shade or Varnish. </p>

**Pigment Density Regulator (Bl) on Other Colors**

<table border="1" id="bkmrk-bay-%2B-no-bl-bay-%2B-bl" style="border-collapse: collapse; width: 100%;"><tbody><tr><td class="align-center" style="width: 49.9383%;">*[![BayDraft_HET_lp_HET_PATN1_HET_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/87Rbaydraft-het-lp-het-patn1-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/87Rbaydraft-het-lp-het-patn1-het-vrn.jpg)*

*Bay + No Bl*

</td><td class="align-center" style="width: 49.9383%;">*[![BayDraft_HET_lp_HET_PATN1_HET_vrn_HET_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/m1nbaydraft-het-lp-het-patn1-het-vrn-het-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/m1nbaydraft-het-lp-het-patn1-het-vrn-het-bl.jpg)*

*Bay + Bl/ and Bay + Bl/Bl*

</td></tr><tr><td class="align-center" style="width: 49.9383%;">*[![BlackDraft_HET_lp_HET_PATN1_HET_vrn.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/blackdraft-het-lp-het-patn1-het-vrn.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/blackdraft-het-lp-het-patn1-het-vrn.jpg)*

*Black + No Bl*

</td><td class="align-center" style="width: 49.9383%;">*[![BlackDraft_HET_lp_HET_PATN1_HET_vrn_HOM_bl.jpg](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/scaled-1680-/blackdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)](https://wiki.huntandjump.com/uploads/images/gallery/2023-11/blackdraft-het-lp-het-patn1-het-vrn-hom-bl.jpg)*

*Black + Bl/ and Black + Bl/Bl*

</td></tr></tbody></table>

<p class="callout info">Pigment Density Regulator altered Bay ever so slightly, but it can be mostly seen in the reduced Varnishing. The expression did not change between HET Bl and HOM Bl. Unlike with DP and Sty, Bl does not change Black Based horses. The Varnish expression stayed the same. Like with Chestnut, Pigment Density Regulator did not change Spot Size. </p>

**Final Observations**

1. Pigment Density Regulator deepens Red Pigment, and can change Chestnut and Bay horses.
2. It has a stronger influence on Chestnut base.
3. Even though it darkens the coat color, it does not change Spot Size.
4. Expression of Appaloosa Varnish will be reduced with Pigment Density Regulator.